streptavidin

BNF-Dextran or BNF-Starch particles are available with streptavidin on the surface for the binding of biotinylated molecules. The matrix of the functionalized particles consists of crosslinked dextran or hydroxyethyl starch. The 100 nm particles can be separated with conventional permanent magnets, while the 80 nm particles have to be separated in high gradient magnetic fields. BNF particles can easily be filtered through 0.22 µm filters. They are supplied in PBS buffer (pH=7.4, 0.02% sodium azide) without any surfactants.

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References
  • Bejhed, R.S., de la Torre, T.Z.G., Donolato, M., Hansen, M.F., Svedlindh, P., Strömberg, M., Bejhed, R.S., de la Torre, T.Z.G., Donolato, M., Hansen, M.F., Svedlindh, P., and Strömberg, M., Turn-on optomagnetic bacterial DNA sequence detection using volume-amplified magnetic nanobeads, Biosensors and Bioelectronics, 2015, 66, 405-11, doi: 10.1016/j.bios.2014.11.048;
  • Bejhed, R.S., Strømme, M., Svedlindh, P., Ahlford, A., and Strömberg, M., Magnetic nanobeads present during enzymatic amplification and labeling for a simplified DNA detection protocol based on AC susceptometry, AIP Advances, 2015, 5(12), 127139;
  • Donolato, M., Antunes, P., Bejhed, R.S., Zardán Gómez de la Torre, T., Østerberg, F.W., Strömberg, M., Nilsson, M., Strømme, M., Svedlindh, P., and Hansen, M.F., Novel readout method for molecular diagnostic assays based on optical measurements of magnetic nanobead dynamics, Analytical chemistry, 2015, 87(3), 1622-9;
  • Donolato, M., Antunes, P., de la Torre, T.Z.G., Hwu, E.-T., Chen, C.-H., Burger, R., Rizzi, G., Bosco, F.G., Strømme, M., and Boisen, A., Quantification of rolling circle amplified DNA using magnetic nanobeads and a Blu-ray optical pick-up unit, Biosensors and Bioelectronics, 2015, 67, 649-55;
  • Engström, A., de la Torre, T.Z.G., Strømme, M., Nilsson, M., and Herthnek, D., Detection of rifampicin resistance in Mycobacterium tuberculosis by padlock probes and magnetic nanobead-based readout, PloS one, 2013, 8(4), e62015;
  • Mezger, A., Fock, J., Antunes, P., Østerberg, F.W., Boisen, A., Nilsson, M., Hansen, M.F., Ahlford, A., and Donolato, M., Scalable DNA-Based Magnetic Nanoparticle Agglutination Assay for Bacterial Detection in Patient Samples, ACS Nano, 2015;
  • Minero, G.A.S., Fock, J., McCaskill, J.S., and Hansen, M.F., Optomagnetic detection of DNA triplex nanoswitches, Analyst, 2017, 142(4), 582-5;
  • Minero, G.A.S., Nogueira, C., Rizzi, G., Tian, B., Fock, J., Donolato, M., Strömberg, M., and Hansen, M.F., Sequence-specific validation of LAMP amplicons in real-time optomagnetic detection of Dengue serotype 2 synthetic DNA, Analyst, 2017, 142(18), 3441-50;
  • Strömberg, M., Zardán Gómez de la Torre, T., Nilsson, M., Svedlindh, P., and Strømme, M., A magnetic nanobead-based bioassay provides sensitive detection of single- and biplex bacterial DNA using a portable AC susceptometer, Biotechnology J, 2014, 9(1), 137-45, doi: 10.1002/biot.201300348;
  • Tian, B., Bejhed, R.S., Svedlindh, P., and Strömberg, M., Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection, Biosensors and Bioelectronics, 2016, 77, 32-9;
  • Tian, B., Ma, J., Zardán Gómez de la Torre, T., Bálint, A.d.m., Donolato, M., Hansen, M.F., Svedlindh, P., and Strömberg, M., Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout, Acs Sensors, 2016, 1(10), 1228-34;
  • Tian, B., Qiu, Z., Ma, J., de la Torre, T.Z.G., Johansson, C., Svedlindh, P., and Strömberg, M., Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry, Biosensors and Bioelectronics, 2016, 86, 420-5;
  • Uddin, R., Burger, R., Donolato, M., Fock, J., Creagh, M., Hansen, M.F., and Boisen, A., Lab-on-a-disc agglutination assay for protein detection by optomagnetic readout and optical imaging using nano-and micro-sized magnetic beads, Biosensors and Bioelectronics, 2016, 85, 351-7;