Streptavidin

BNF-Dextran- und BNF-Starch-Partikel werden mit Streptavidin auf der Oberfläche für die Bindung von biotinylierten Molekülen angeboten. Die Partikel besitzen eine Matrix aus vernetztem Dextran bzw. vernetzter Hydroxylethyl-Stärke. Die 100 nm – Partikel können mit konventionellen Permanentmagneten separiert werden, während die 80 nm – Partikel vorzugsweise im Hochgradientenmagnetfeld separiert werden. Die BNF-Partikel können einfach durch 0,22 µm-Filter filtriert werden und werden als Suspension in PBS-Puffer (pH=7,4 , 0,02% Natriumazid) ohne Zusatz von Detergenzien geliefert.

Es werden 4 Ergebnisse angezeigt

Referenzen
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  • J. Blomgren, F. Ahrentorp, D. Ilver, C. Jonasson, S. Sepehri, A. Kalaboukhov, D. Winkler, T. Zardán Gómez de la Torre, M. Strømme and C. Johansson, Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method, Nanomaterials, 2018, 8(11), 887;
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  • M. Donolato, P. Antunes, R. S. Bejhed, T. Zardán Gómez de la Torre, F. W. Østerberg, M. Strömberg, M. Nilsson, M. Strømme, P. Svedlindh and M. F. Hansen, Novel readout method for molecular diagnostic assays based on optical measurements of magnetic nanobead dynamics, Analytical chemistry, 2015, 87(3), 1622–1629;
  • M. Donolato, P. Antunes, T. Z. G. de la Torre, E.-T. Hwu, C.-H. Chen, R. Burger, G. Rizzi, F. G. Bosco, M. Strømme and A. Boisen, Quantification of rolling circle amplified DNA using magnetic nanobeads and a Blu-ray optical pick-up unit, Biosensors and Bioelectronics, 2015, 67(649–655;
  • A. Engström, T. Z. G. de la Torre, M. Strømme, M. Nilsson and D. Herthnek, Detection of rifampicin resistance in Mycobacterium tuberculosis by padlock probes and magnetic nanobead-based readout, PloS one, 2013, 8(4), e62015;
  • S. W. Gordon-Wylie, C. Grüttner, H. Teller and J. B. Weaver, Using magnetic nanoparticles and protein/protein interactions to measure pH at the nanoscale, IEEE Sensors Letters, 2020, 1–1;
  • H. Khurshid, Y. Shi, B. L. Berwin and J. B. Weaver, Evaluating blood clot progression using magnetic particle spectroscopy, Medical physics, 2018, 45(7), 3258–3263;
  • G. A. S. Minero, J. Fock, J. S. McCaskill and M. F. Hansen, Optomagnetic detection of DNA triplex nanoswitches, Analyst, 2017, 142(4), 582–585;
  • G. A. S. Minero, C. Nogueira, G. Rizzi, B. Tian, J. Fock, M. Donolato, M. Strömberg and M. F. Hansen, Sequence-specific validation of LAMP amplicons in real-time optomagnetic detection of Dengue serotype 2 synthetic DNA, Analyst, 2017, 142(18), 3441–3450;
  • F. W. Østerberg, G. Rizzi, M. Donolato, R. S. Bejhed, A. Mezger, M. Strömberg, M. Nilsson, M. Strømme, P. Svedlindh and M. F. Hansen, On-Chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii Spores and Vibrio Cholerae, Small, 2014, 10(14), 2877–2882;
  • M. Strömberg, T. Zardán Gómez de la Torre, M. Nilsson, P. Svedlindh and M. Strømme, A magnetic nanobead-based bioassay provides sensitive detection of single- and biplex bacterial DNA using a portable AC susceptometer, Biotechnology Journal, 2014, 9(1), 137–145;
  • B. Tian, R. S. Bejhed, P. Svedlindh and M. Strömberg, Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection, Biosensors and Bioelectronics, 2016, 77(32–39;
  • B. Tian, Y. Han, E. Wetterskog, M. Donolato, M. F. Hansen, P. Svedlindh and M. Strömberg, MicroRNA Detection through DNAzyme-Mediated Disintegration of Magnetic Nanoparticle Assemblies, J ACS sensors, 2018, 3(9), 1884–1891;
  • B. Tian, X. Liao, P. Svedlindh, M. Strömberg and E. Wetterskog, Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA, ACS sensors, 2018, 3(6), 1093–1101;
  • B. Tian, J. Ma, T. Zardán Gómez de la Torre, A. d. m. Bálint, M. Donolato, M. F. Hansen, P. Svedlindh and M. Strömberg, Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout, Acs Sensors, 2016, 1(10), 1228–1234;
  • B. Tian, Z. Qiu, J. Ma, T. Z. G. de la Torre, C. Johansson, P. Svedlindh and M. Strömberg, Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry, Biosensors and Bioelectronics, 2016, 86(420–425;
  • R. Uddin, R. Burger, M. Donolato, J. Fock, M. Creagh, M. F. Hansen and A. Boisen, Lab-on-a-disc agglutination assay for protein detection by optomagnetic readout and optical imaging using nano-and micro-sized magnetic beads, Biosensors and Bioelectronics, 2016, 85(351–357;