Ni-NTA

Sicastar®-M particles with a diameter of 1.5 µm are offered with the nickel(II)-nitrilotriacetic acid complex (Ni-NTA) for the direct binding of histidine labeled proteins.

Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni2+ ion immobilized on a matrix and the histidine side chains. Following washing of the matrix 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.

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References
  • Hughes, S., McBain, S.C., Dobson, J., and El Haj, A.J., Selective activation of mechanosensitive ion channels using magnetic particles, J R Soc Interface, 2008, 5, 855-63;