NTA

Sicastar®-M particles with a diameter of 1.5 µm are offered with the nickel(II) chelator nitrilotriacetic acid (NTA) for the binding of histidine labeled proteins.

Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni2+ ion immobilized on a matrix and the histidine side chains. Following washing of the matrix 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.