Ni-NTA

micromer® particles can be offered with the nickel(II)-nitrilotriacetic acid complex (Ni-NTA) for the binding of histidine labeled proteins in the size range of 1 µm to 10 µm. Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni2+ ion immobilized on the particle matrix and the histidine side chains. Following washing of the matrix 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.

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References
  • C. O. Mejean, A. W. Schaefer, E. A. Millman, P. Forscher and E. R. Dufresne, Multiplexed force measurements on live cells with holographic optical tweezers, Opt. Express., 2009, 17(8), 6209– 6217;