NTA

sicastar® particles are offered with the nickel(II) chelator nitrilotriacetic acid (NTA) or ready to use with the corresponding nickel complex (Ni-NTA) for the binding of histidine labeled proteins in the size range of 300 nm to 20 µm. They are supplied in water. Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni2+ ion immobilized on a matrix and the histidine side chains. Following washing of the matrix 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.
The particles are supplied in water without any surfactants.

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References
  • D. M. Suter, A. W. Schaefer and P. Forscher, Microtubule dynamics are necessary for src family kinase-dependent growth cone steering, Current Biology, 2004, 14(1194–1199;