NTA

The cluster-typed sicastar®-M-CT particles with a mean diameter of 6 microns are offered with the nickel(II) chelator nitrilotriacetic acid (NTA) for the binding of histidine labeled proteins.

Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni2+ ion immobilized on a matrix and the histidine side chains. Following washing of the matrix 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.

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