Ni-NTA

The cluster-typed sicastar®-M-CT particles with a mean diameter of 6 microns are offered with the nickel(II)-nitrilotriacetic acid complex (Ni-NTA) for the direct binding of histidine labeled proteins.

Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni2+ ion immobilized on a matrix and the histidine side chains. Following washing of the matrix 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.

Showing the single result