NH2

Perimag® particles with a diameter of 130 nm are designed with amino groups on the surface for the covalent binding of proteins, antibodies or other molecules. They consist of about 70% (w/w) iron oxide in a matrix of cross-linked dextran (40.000 Da). The perimag® particles cannot be separated with conventional permanent magnets, but in a high gradient magnetic field. The functionalized perimag® particles are supplied in water without any surfactants. Thus several conjugation methods can be directly applied, e.g. maleimide or SPDP functionalization of the amino groups for conjugation of SH-labeled biomolecules (see Technote 201 and Technote 202).

Perimag® particles with amino groups on the surface display a direct uptake by stem cells without the use of any transfection agents. Therefore they can be used for homing and tracking of stem cells in regenerative medicine (Steinke 2016).

Showing the single result

References
  • Drews, L.B., Croft, L.R., Kosuge, H., Saritas, E.U., Goodwill, P.W., McConnell, M.V., Conolly, S.M., and Tirrell, M.V., Imaging atherosclerotic plaques in vivo using peptide-functionalized iron oxide nanoparticles, Magnetic Particle Imaging (IWMPI), 2013 International Workshop on, 2013, 1-;
  • Kilian, T., Fidler, F., Kasten, A., Nietzer, S., Landgraf, V., Weiß, K., Walles, H., Westphal, F., Hackenberg, S., Grüttner, C., and Steinke, M., Stem cell labeling with iron oxide nanoparticles: impact of 3D culture on cell labeling maintenance, Nanomedicine, 2016, 11(15), 1957-70;