NTA

nanomag®-D particles with diameters of 130 nm, 250 nm and 500 nm are offered with the nickel(II) chelator nitrilotriacetic acid (NTA) for the binding of histidine labeled proteins. Recombinant proteins containing a 6xhistidine-tag can be purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography which is based on the interaction between a transition Ni 2+ ion immobilized on a matrix and the histidine side chains. Following washing of the matrix by separation with a permanent magnet the 6xhistidine-tag fusion proteins can be eluted by adding free imidazole or EDTA or by reducing the pH.

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